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These advanced methods can provide more reliable data without causing unnecessary suffering to animals

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Furthermore, vaping typically produces less odour and fewer environmental pollutants than smoking, making it a more socially acceptable option for many especially as there is limited evidence of second hand vaping, compared with secondhand smoke that we know to be a health risk (4)

Electron microscopic analysis of the mucosa of the ET in the control group revealed goblet cells and pseudostratified mucociliary respiratory-like epithelium with cilia (Fig